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Evaluation of characteristics of cell sheet in culture medium using air puff


Regenerative medicine is becoming popular all over the world, especially, cell sheet for damaged cornea is expected to be practical available in the near future. As for cultivative procedure, it is important to judge whether cell sheet is enough cultivated or not. So far, this qualitative judgement has been done by specialists without any quantative evaluation. This paper is to challenge to evaluate the cultivative level quantitatively. To do this, there are three points to be considered; (1)cell sheet is sensitive for being dry, (2)cell sheet is thin with its thickness of less than 100[um], and (3) chell sheet is transparent. Under these constraints, we found that there is a significant difference between well caltivated and injurely caltibated, especially in shed capability.

Cell sheet evaluation using air puff